Scanning Electron Microscopic Studies of Tissue Elastin Components Exposed by a KOH-Collagenase or Simple KOH Digestion Method.
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چکیده
منابع مشابه
Scanning electron microscopic studies of tissue elastin components exposed by a KOH-collagenase or simple KOH digestion method.
A KOH-collagenase or simple KOH digestion method was employed for scanning electron microscope (SEM) studies of elastin components in the rat thoracic aorta, mouse urinary bladder, and human ductus deferens. Immersion of the fixed tissues in 30% KOH solution for 8-10 min at 60 degrees C, with or without subsequent collagenase treatment, successfully removed collagen fibrils and basal laminae wh...
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Certain parts of the peripheral nervous system were observed by a modification of the KOH-collagenase method by Miller et al. (1982). The sciatic nerve of the mouse, and dorsal root ganglion, lingual muscle and jejunum of the rat were fixed over one day at room temperature in a fixative containing paraformaldehyde and glutaraldehyde. After fixation, specimens were treated with 5N KOH solution f...
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ژورنال
عنوان ژورنال: Archives of Histology and Cytology
سال: 1991
ISSN: 0914-9465,1349-1717
DOI: 10.1679/aohc.54.427